摘要
目的:探讨肺硬化性血管瘤(PSH)整个病变的克隆性并阐明其本质.方法:利用激光显微切割技术获取22例女性肺PSH组织中多角形细胞和表面立方细胞构成的整个病变及病变周围正常肺组织,分别提取基因组DNA,通过基于女性体细胞构成组织内X染色体失活嵌合性和磷酸甘油酸激酶(PGK)基因位点的克隆性分析、观察PSH整个病变的克隆性.结果:22例肺PSH光镜下均表现为典型的组织病理学特征,即呈实性、乳头状、硬化性和出血性组织结构.病变主要由间质圆形或多角形细胞及被覆在乳头状结构表面的立方细胞所构成.克隆性检测结果表明有7例31.8%(7/22)显示PGK基因位点多态性.经HpaⅡ酶切后,所有具有多态性的标本2个条带中有1个条带的密度明显减弱或消失,而另1个条带保留,证明其均为肿瘤性增生.结论:PSH是一种真性肿瘤,构成PSH的2种主要细胞起源于同一类型细胞.
AIM： To investigate the clonal status of the whole lesion of pulmonary scleresing haemangioma （PSH）. METHODS ： laSH whole lesions composed of polygonal ceils and surface cuboidal cells, and the surrounding normal lung tissues were microdissected from routine hematoxylin and eosin-stained sections, respectively in 22 female patients. Genomic DNA was extracted, pretreated with methylation-sensitive restrictive endonuclease Hpa Ⅱ, and then amplified via nested polymerase chain reaction（PCR） for phosphoglycerate kinase gene. Clonal analysis was conducted based on X-chromosomal inactivation mosaicism and polymorphism at PGK loci in female somatic cells and the clonality of the PSH whole lesion was observed. RESULTS： Microscopically, typical histopathological characteristics, including solid, papillary, sclerotic and hemorrhagic patterns, were observed in all the 22 cases. The lesions were composed of round or polygonal cells and surface cuboidal cells. Clonality assay showed that the rate of ploymorphism was 31. 8% （7/22） for PGK gene. Following digestion by Hpa II, one of the two bands in all the samples disappeared, while the other band reserved, suggesting their neoplastic nature. CONCLUSION： The PSH whole lesion is a real neoplastic lesion. Its two kinds of cells originate from a common type of cell.
出处
《第四军医大学学报》
北大核心
2009年第17期1594-1596,共3页
Journal of the Fourth Military Medical University
关键词
单克隆
硬化性血管瘤
肺肿瘤
磷酸甘油酸激酶
monoclonal
sclerosing haemangioma
lung neoplasms
phosphoglycerate kinase
