摘要
目的 研究产地和季节对滇白珠木脂素苷 (L G)含量的影响 ,为开发提供依据。方法 高效液相色谱法。色谱柱为 Kromasil C1 8(0 .4cm× 2 5 cm) ,流动相为甲醇 -乙腈 -水 (3 0∶ 5∶ 65 ) ,波长 2 2 0 nm,外标法。结果 L G含量在滇白珠内分布规律是 :根 >茎 >叶 ,只有楚雄林下样品茎高于根。采自海南、广东、云南、贵州、重庆等省市的 9个地点的样品中 ,根中 L G含量 (mg/g)最高的是海南 2 .15 ,其次是贵州 2 .0 0 ,云南楚雄和昆明样品仅 1.42和1.44。根中 LG含量在 9月 (果期 )以前仅 1.43 8mg/g,比 11月低 2 4.4%。结论 海南和贵阳样品 LG含量最高 ,但结合生物量考虑 ,选云南大理和广西金秀的资源更合理。不同季节滇白珠 LG含量变化规律是 :花期 (6～ 7月 )和果期 (8～ 9月 )根中含量较低 ,因此采根应避免花果期。而茎则花果期含量较高 ,故采茎应选花期。
Object To study the content of lignan glycosides (LG) in Dian Bai Zhu Gaultheria leucocarpa var. yunnanensis (Franch.) T.Z.Hsu & R.C.Fang] from different productive area and growth period, so as to provide the basis for development of the plant. from different productive area and growth period, so as to provide the basis for development of the plant. Methods The LG content in Dian Bai Zhu was determined by HPLC with Kromasil C 18 column (0.4 cm × 25 cm), mobile phase is methanol acetonitrile water (30∶5∶65), determinant wavelength is 220 nm. Results The distribution of the LG content in most plants was root> stem >leaf. The only LG content in Chuxiong samples is stem > root. The comparison of the LG content in nine samples from Hainan, Guangdong, Yunnan, Guizhou and Chongqing suggested that the LG content in the root of the plant from Hainan was the highest, 2.15 mg/g; the LG in sample from Guizhou was the second, 2.00 mg/g; the LG in samples from Chuxiong and Kunming is the least as 1.42 mg/g and 1.44 mg/g, respectively. The LG content in root was 1.438 mg/g before September which was less than that in November by 24.4%. Conclusion The taking resources from Dali, Yunnan and Jinxiu, Guangxi as medicine are appropriately considered their biomass, though the LG content in the plants from Hainan and Guiyang was the highest. The plant root should be harvested after September, because the LG content in root is relatively low before this month; the stem should be harvested during flowering and fruit period (from June to September), the whole plant is best harvested during August.
出处
《中草药》
CAS
CSCD
北大核心
2002年第4期353-355,共3页
Chinese Traditional and Herbal Drugs
关键词
产地
季节
滇白珠
木脂素苷
含量
影响
高效液相色谱法
Gaultheria leucocarpa var. yunnanensis (Franch.) T.Z.Hsu & R.C.Fang
contents of lignan glycosides (LG)
HPLC
