摘要
目的 观察水杉总黄酮 (FMG)对大鼠血小板聚集活性、血小板 5 -HT释放反应、血浆 NO含量及血液流变性的影响。方法 比浊法测定大鼠血小板聚集活性、荧光光度法测定血小板 5 -HT释放反应、分光光度法测定血浆 NO含量、血液流变全自动分析仪检测血液流变性。结果 FMG抑制大鼠血小板聚集活性 ,抑制血小板 5 -HT释放 ,升高血浆 NO含量 ,降低急性血瘀大鼠的全血比粘度、血浆比粘度、红细胞电泳时间、红细胞压积、红细胞计数、血沉方程 K值 ,改善大鼠的血液流变性。结论 FMG有抗血小板聚集活性、改善血液流变性的作用。其抗血小板聚集的作用机制可能与抑制血小板释放反应、增加体内 NO合成及 Ca2
Object To observe the effect of total flavones isolated from Metasequoia glyptostroboides Hu et Cheng (FMG) on platelet aggregation, 5 HT of release, content of NO in plasma and hemorrheological parameters in rats. Methods Platelet aggregation was quantified by turbidimetry, release of 5 HT was assayed by fluorescence technique, and content of NO in plasma was determined by spectrophotometry. Results Inhibition of platelet aggregation and release of 5 HT, increase of NO content in plasma, as well as decrease of whole blood viscosity, plasma viscosity, red cell electrophoresis time, red cell specific volume, red cell count and K value in ESR equation were observed in the acute blood stasis rat, thereby the rat's hemorrheological properties were improved. Conclusion FMG is able to inhibit the platelet aggregation and improve the hemorrheological parameters in acute blood stasis rat. The inhibition of release of 5 HT, increase of NO content in plasma and antagonistic action of Ca 2+ may be the mechanism of its actions.
出处
《中草药》
CAS
CSCD
北大核心
2002年第4期327-329,共3页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助课题 ( N o.39970 0 85)
关键词
水杉总黄酮
大鼠
血小板聚集
血液流变性
5-HT释放反应
一氧化氮
中药
total flavones of Metasequoia glyptostroboides Hu et Cheng (FMG)
platelet aggregation
5 HT release reaction
NO
hemorrheological properties
