摘要
目的 探讨乳腺珠蛋白 (hMAM )mRNA在乳腺癌患者外周血中的表达及临床意义。方法 采用逆转录 聚合酶链方法检测 5 6例乳腺癌、8例乳腺增生和 8例正常乳腺者外周血中hMAMmRNA的表达 ,并比较了hMAMmRNA表达与乳腺癌生物学特性的关系 ,术后早期化疗对hMAMmRNA表达的影响。 结果 5 6例乳腺癌患者外周血中hMAMmRNA表达阳性率为 30 4 % (17/ 5 6 ) ,8例慢性乳腺增生者和 8例正常乳腺者hMAMmRNA表达均为阴性 ,2组hMAMmRNA表达差异有非常显著性意义 (χ2 =19 76 6 ,P <0 0 1)。hMAMmRNA的表达与患者年龄、原发癌灶大小 ,临床分期差异无显著性意义 (χ2 =1 2 5 6 ,P >0 0 5 )。 17例hMAMmRNA表达阳性者经术后早期短程化疗后 ,转阴率为4 1 2 % (7/ 17) ,手术前hMAMmRNA表达阴性者无一例表达阳性。 结论 hMAMmRNA特异表达于乳腺癌患者外周血 ,有可能成为诊断乳腺癌和判断预后的一个独立指标。
Objective To study the expression of human mammaglobin (hMAM) mRNA in the peripheral blood of breast cancer patients and its implication. Methods The expression of human mammaglobin mRNA was determined by using RT PCR method in 56 patients with peripheral blood breast cancer, 8 patients with breast hyperplasia and 8 women with normal breast. The expression of hMAM mRNA was compared with biological significance and change of hMAM mRNA in chemotherapy after operation. Results The expression of hMAM mRNA was negative in 8 patients with breast hyperplasia, 8 women with normal breast and 56 patients with breast cancer, The positive rate was 30 4%(17/56) (χ 2=19 766, P <0 01).The expression of hMAM mRNA in peripheral blood was not correlated with clinical stage,primary tumor size and patients age (χ 2=1 256, P >0 05).After short term large dose of chemotherapy, 41 2%(7/17) patients turned positive before operation to negative hMAM mRNA expression and negetive expression before operation positive expression after chemotherapy. Conclusions This study suggests that hMAM is sensitive and specific for breast cancer. Detection of the expression of hMAM mRNA in peripheral blood of breast cancer is of value in the diagnosis and judgement of prognosis of breast cancer.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2002年第3期168-170,共3页
Chinese Journal of Surgery
