摘要
为研究不同遮阴处理下皂苷合成关键酶表达对珠子参总皂苷合成与积累的影响,以全光照、30%遮阴和50%遮阴3种处理下的盆栽珠子参为材料,分别用实时荧光定量PCR法和紫外分光光度法测定了不同生长时期珠子参叶和根茎中3个关键酶基因(CAS,DS,β-AS)的表达和总皂苷含量。结果表明,在开花期CAS,DS,β-AS均在珠子参地上部分高表达,30%遮阴处理显著抑制了叶中CAS的表达而促进了茎,叶和花中DS,β-AS的表达,推测在此时期皂苷合成的主要部位为叶。整个生长周期内,DS,β-AS的表达与叶中总皂苷含量的变化均呈现出"低-高-低"的趋势,相关系数分析表明,两者之间为正相关关系。遮阴处理下DS,β-AS的总体表达水平和总皂苷含量远高于全光照处理,其中以30%遮阴处理对总皂苷积累的促进作用最明显。因此建议在人工栽培珠子参时,采取30%的遮阴处理,以利于珠子参皂苷的积累和品质的提升。
To explore the effects of shading and the expression of key enzyme genes on the synthesis and accumulation of Panax japonicus var. major saponins, different shading treatments(0%, 30%,50%) of potted P. japonicus var. major were used as test materials, the expression of three key enzyme genes(CAS,DS,β-AS)of leaves and rhizomes in different growth periods of P. japonicus var. major was determined by real-time quantitative PCR, the content of total saponins was determined by ultraviolet spectrophotometry. The results indicated that, in flowering stage, CAS,DS,β-AS were highly expressed in the aerial parts of P. japonicus var. major, 30% shading treatment significantly inhibited the expression of CAS in leaves and promoted the expression of DS and β-AS in stems, leaves and flowers, it was speculated that the main part of saponin synthesis was leaf in this stage. Both the expression levels of DS and β-AS and changes in the content of total saponins in leaves showed a tendency of low-high-low throughout the growth cycle, correlation coefficient analysis showed that there was a positive correlation between them. Compared with control, the expression levels of DS and β-AS and the content of total saponins were greatly enhanced under shading treatment, 30% shading treatment significantly promoted the accumulation of total saponins. Therefore, it is suggested that 30% shading treatment should be applied to the artificial cultivation of P. japonicus var. major, which is beneficial to the accumulation and quality improvement of saponins.
作者
黄文静
孙晓春
李铂
史鑫波
岳正刚
蔡兴航
杨洁
唐志书
HUANG Wen-jing;SUN Xiao-chun;LI Bo;SHI Xin-bo;YUE Zheng-gang;CAI Xing-hang;YANG Jie;TANG Zhi-shu(Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine,Xianyang 712083,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第19期3855-3861,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81703652)
现代农业产业技术体系建设专项资金项目(CARS-21)
陕西省社会发展科技攻关项目(2015SF300)
关键词
珠子参
遮阴
基因表达
三萜皂苷
Panax japonicus var.major
shading
gene expression
triterpenoid saponins
