摘要
目的 :探讨人参皂甙Rg1对多巴胺诱导PC12细胞凋亡的保护作用及可能机制。 方法 :采用PC12细胞为模型 ,以不同浓度的DA来诱导细胞凋亡的发生 ,用流式细胞仪检测PC12细胞的亚二倍体峰 ,按Griess法测定NO代谢产物NO2 ·的浓度 ,并观察人参皂甙Rg1(10 μmol/L)预防性给药对其影响。 结果 :0、0 15、0 30、0 45和 0 6 0mmol/LDA处理组的凋亡率分别为 1 6 1±0 18、40 6 0± 1 74、46 6 7± 1 45、5 4 49± 1 6 1和 6 4 39± 1 6 0 % ,与Rg1预防组 0 73± 0 11、1 16± 0 0 9、1 35± 0 0 7、1 5 3± 0 10和 1 6 1± 0 12 %比较均有显著性差异 (P <0 .0 1) ;DA处理组的NO2 ·浓度分别为 1 82± 0 0 5、3 5 7± 0 0 2、8 82± 0 0 4、18 0 0± 0 0 7和 2 7 34± 0 0 9μmol/L ,与Rg1预防组的 1 0 0± 0 0 7、1 36± 0 0 7、1 2 5± 0 0 4、3 39± 0 11和 3 82± 0 11μmol/L比较亦都有显著性差异 (P <0 .0 1)。结论 :NO生成增多可能是DA诱导PC12细胞凋亡的重要信号分子 ,人参皂甙Rg1可抑制NO生成 。
Objective: To explore the protective effect and possible mechanism of Ginsenoside Rg1 on PC12 cells apoptosis induced by dopamine. [WT5”HZ]Methods:[WT5”BZ] In this study, we used PC12 cells as cell model. Apoptosis was induced by DA at the ranges of the contents of 0、0 15、0 30、0 45 and 0.60mmol/L, respectively. Here we detected the subdiploid peak with flow cytometry. Nitrite was quantified by the Griess reaction. The effects of Ginsenoside Rg1 on PC12 cells were examined through to detect the subdiploid peak and nitrite. [WT5”HZ]Results:[WT5”BZ] The ratios of apoptosis induced by DA were respectively 1 61±0 18、40 60±1 74、46 67±1 45、54 49±1 61 and 64 39±1 60%,significantly higher than those of the Rg1 protective group (0 73±0 11、1 16±0 09、1 35±0 07、1 53±0 10 and 1 61±0 12%,P<0.01). The levels of nitrite (1 82±0 05、3 57±0 02、8 82±0 04、18 00±0 07 and 27 34±0 09umol/L) in DA group were significantly higher than those of the Rg1 protective group (1 00±0 07、1 36±0 07、1 25±0 04、3 39±0 11 and 3 82±0 11μmol/L%, respectively, P<0.01). [WT5”HZ]Conclusion:[WT5”BZ] The high levels of NO formation might be a important signaling molecule that DA induced PC12 cells apoptosis. The Ginsenoside Rg1 could inhibit NO formation and prevent PC12 cells from apoptosis induced by DA.
出处
《中药药理与临床》
CAS
CSCD
2000年第2期13-15,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
福建省自然科学基金重点课题资助项目
