摘要
目的探讨人参皂苷Rg1对脑缺血-再灌注大鼠海马CA1区神经元凋亡的影响。方法60只健康大鼠随机分为假手术组,模型组,人参皂苷Rg110、20、40mg/kg组,尼莫地平组,每组10只。采用线栓法制作大脑中动脉栓塞(MCAO)模型。术后24h采用电镜及TUNEL法对海马CA1区的神经元凋亡情况进行检测。结果模型组部分神经元坏死,可见神经元凋亡,有时有凋亡小体形成。Rg1各组与模型组比较,Rg1各组海马CA1区粗面内质网肿胀及线粒体嵴断裂均有不同程度的减轻,凋亡细胞数量显著降低(P<0.05)。Rg140mg/kg组凋亡细胞数低于尼莫地平组(P<0.05),尼莫地平组凋亡细胞数低于10mg/kg组(P>0.05)与20mg/kg组相当。结论人参皂苷Rg1可以通过干预神经元凋亡过程发挥对脑缺血再灌注损伤的保护作用。
Objective To explore the roles of ginsenoside Rgl on neurons apoptosis caused by cerebral ischemic-reperfusion in hippoeampus CA1 of rat. Methods 60 healthy rats were randomly divided into： sham, model, GinsenosideRgltreated and nimodiping treated groups. 24h After middle cerebral artery oeelusion（MCAO）, the apoptosis status and the ultramicrostructure of neurons in hippocampus CA1 zone were detected by TUNEL assay and transmission electric microscopy respectively. Results Apoptotie neurons were observed in hippoeampus CA1 zone in model group, but not in sham. Less viable apoptotie cells were observed in hippocampus CA1 zone and the extent of rER edema and crista mitoehondria fracture decreased in hippocampus CA1 zone in Ginsenoside Rgl treated group compared with model group.The percentage of apoptosis neurons was significantly decreased in Rgl 40 mg/kg treated group than in nimodiping treated group（P〈0.05） which was lower than Rgl 10 mg/kg treated group and similar to Rgl 20 mg/kg treated group. Conclusion Ginsenoside Rgl inhibited significantly the apoptosis of neurons in ischemic zone.
出处
《解剖科学进展》
CAS
2009年第4期376-379,共4页
Progress of Anatomical Sciences
