摘要
小鼠脾细胞体外培养３ｈ即可检测到ＩＬ１αｍＲＮＡ的表达，４ｈ达到高峰，５ｈ后降至不能检出的水平。ＴＮＦαｍＲＮＡ在４ｈ开始出现，４．５ｈ时达到高峰，５．５ｈ后逐渐消失。在培养开始时分别加入灵芝多糖ＧＬＢ７５、１０、２０、４０μｇ／ｍｌ，４ｈ及４．５ｈ分别检测ＩＬ１α及ＩＬ１αｍＲＮＡ的表达情况。结果ＧＬＢ７能促进ＩＬ１α及ＩＬ１αｍＲＮＡ的表达，对ＩＬ１αｍＲＮＡ的表达提高率分别为７．６％、６７．３％、１０５．０％、１４３．１％；对ＴＮＦαｍＲＮＡ的表达提高率分别为９．９％、３３．９％、４５．９％、７５．８％。８ｈ后ＧＬＢ７对培养上清中ＩＬ１活性的提高率分别为１．４％、３．０％、６．７％、８．１％；１２ｈ后对培养上清中ＴＮＦα活性的提高率分别为３．０％、１４．１％、２８．２％、３５．７％。结果说明灵芝多糖ＧＬＢ７能通过促进小鼠脾细胞原代培养时ＩＬ１α及ＴＮＦαｍＲＮＡ的表达从而促进ＩＬ１及ＴＮＦα的合成与分泌。
Interleukin1(IL1)α mRNA expression could be detected in murine splenocytes after 3h incubation,it reached the peak level at 4h and then declined to be undetected after 5h incubation.TNF(Tumor Necrosis Factor)α mRNA expression occured after 4h incubation,reached the peak level at 4.5h and disappeared gradually after 5.5h.Ganoderma polysaccharide (GLB7) in different final concentrations(5,10,20,40μg/ml) was added into the cultures at the beginning of incubation and then,the amount of mRNA expressions for IL1α and TNFα was determined after 4h and 4.5h respectively.It was found that GLB7 could promote the mRNA expressions for both IL1α and TNFα,the increase rates of mRNA expression for IL1α were 7.6%,67.3%,105.0% and 143.1%,and were for TNFα 9.9%,33.9%,45.9% and 75.8% respectively.After 8h incubation,GLB7 could increase the activity of IL1 in the supernatants by 1.4%,3.0%,6.7% and 8.1%,and raise the activity of TNFα by 3.0%,14.1%,28.2% and 35.7% after 12h incubation respectively.It is suggested that the enhancement effects of Ganoderma polysaccharide on the production and secretion of IL1 and TNFα in primary murine splenocyte culture are due to promoting of the mRNA expressions for IL1α and TNFα respectively.
出处
《中药药理与临床》
CAS
CSCD
1998年第2期16-18,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金
