摘要
研究了草鱼在不同水温条件下受抗原刺激后其中和抗体的变化。15℃培养条件下中和抗体上升缓慢,9周内滴度低于1:8;20℃时,3周后抗体可上升到1:256,最高达1:5270,而在25℃时,1周中和抗体即达到1:570,最高可达1:20000以上。并探索了从草鱼血清中提纯抗体的条件,研究其抗体的特性。草鱼血清中的抗体为大分子蛋白,容易解离为抗原性相同,分子量近似于人IgG的较小分子,含有较多的二硫键,具有类似IgM的某些特性。
Water temperature plays an important role in the antibody production in grass carp. At 15℃, the titer of neutralizing antibody developed very slowly and was less than 1:8 when the grass carp had been reared for 9 week; In contrast, at 20℃ the titer of antibody was 1: 256 three weeks after infection and reached the highest titer of 1:5 270 after 6 weeks; The fish reared at 25℃ showed a level of antibody of 1:570 in one week and the highest titer was more than 1:20, 000. The antibody in grass carp serum is a kind of macroglobulin. it breaks down easilv into smaller polypeptides, which have the same antigenicity as that for the antibody and are similar to human IgG in molecular weight. The results indicated that the antibody of grass carp possesses some characteristics of human IgM.
出处
《水生生物学报》
CAS
CSCD
北大核心
1991年第4期321-326,共6页
Acta Hydrobiologica Sinica
关键词
草鱼
免疫应答
抗体
Immune response
Humoral immunity
Antibody
IgM of Grass carp
