摘要
本文报道抗体、补体、补体加抗体对机械转变日本血吸虫童虫的杀伤作用,并比较其杀伤效率。用光学显微镜及扫描电镜观察形态变化。结果显示杀伤率为补体加抗体>单纯补体>单纯抗体。除去补体血清中的B因子,杀伤率明显下降,说明补体C_3旁路途径参与杀伤童虫的作用。童虫孵育于含有补体及抗体的培养基中72h后,虫体头背部体棘零乱或消失,有些体被破损及脱皮。
This paper reports the damage effect of immune serum and complement on mechanically transformed schistosomula of schistosoma japonicum. The schistosomula were incubated in the media which contained sera in different dilutions, complement, and serum with complement. Normal serum was used as control. The plates were then incubated at 37℃ in 5%CO_2 incubator for 4, 16, 24, 48, 72, and 96 h. The activity of the schistosomula became low and some of the worms died after being incubated in fresh immune serum for 16 h. The mortality increased with the time of incubation. The mortality of the schistosomula was 58.3%, 58.5%, and 77.3% respectively after 96h in the media in which the ratio of medium to immune serum was 1:1, 1:2, and 1:9. In the medium with inactivated normal serum, the activity of the schistosomula was relatively normal and the dead worms were seldom seen. After the schistosomula were incubated for 4h in the medium which contained complement, the bubble-like substances appeared on the body surface of schistosomula. After 48 h, the body of worms wrinkled obviously. Some opaque particles appeared on the surface of the schistosomula. The mortality of the worms was 86.1% after 96 h. The mortality of the schistosomula in the medium in which B factor in serum had been eliminated was lower than that in the medium with fresh guinea pig serum. The highest mortality of the schistosomula occurred in the medium with immune serum and complement and was 98.13% when the schistosomula were incubated for 96h. The schistosomula in the medium with immune serum and complement were seriously damaged, as observed under scanning electron microscope. The spines on the body surface became inordinately arrayed or disappeared. Some part of the body surface was broken and stripped. The damage mechanism of the immune factors in the serum of the schistosomula is discussed.
出处
《水生生物学报》
CAS
CSCD
北大核心
1991年第1期73-78,共6页
Acta Hydrobiologica Sinica
基金
国家自然科学基金
关键词
日本血吸虫
童虫
抗体
补体
Schistosoma japonicum
Schistosomulutn
Antibody
Complement
Immune serum
