摘要
用对应于戊型肝炎病毒（ｈｅｐａｔｉｔｉｓＥｖｉｒｕｓ，ＨＥＶ）开读框架ＯＲＦ１、ＯＲＦ２和ＯＲＦ３部分氨基酸序列的三段多肽为抗原，以鼠伤寒沙门菌体为载体，经静脉免疫家兔和经腹腔免疫ＢＡＬＢ／ｃ小鼠，制备出抗－ＨＥＶ免疫血清。两次加强免疫后，经本室研制的抗－ＨＥＶ ＩｇＧＥＬＩＳＡ试剂盒检测，１：１０稀释血清Ａ值达１．５０～２．００。用沙门菌和ＨＥＶ多肽分别做中和试验，ＨＥＶ多肽的中和抑制率大于５０％，而沙门菌不能中和血清中的抗体。用此免疫血清与抗－ＨＥＶＩｇＣ阳性的病人血清做阻断试验，阻断率大于５０％。上述结果证明家兔和小鼠产生的抗体为特异性抗－ＨＥＶ抗体。
Three synthetic peptides corresponding to HEV genome OR1、ORF2 and ORF3,combined with SalmonelIa typhimurium were used as antigen to immunize rabbit and BALB / cmice. After two boost doses,the absorbance value of 1:10 diluted serum was 1.50-2.00 in thespecific ELISA test.HEV peptides could neutralize the antibodv at the rate of more than 50 %,whereas the Salmonella could not neutralize it.In addition to that.the immunized serum couldblock the conjugation of anti-HEV antibody from hepatitis E patients with specific HEV antigenat the rate of more than 50%These results show that the antibody produced by rabbit or mice isspecific anti-HEV antibodv.
出处
《军事医学科学院院刊》
CSCD
北大核心
1995年第3期168-169,共2页
Bulletin of the Academy of Military Medical Sciences
关键词
戊型肝炎病毒
氨基酸序列
鼠伤寒沙门菌
制备
hepatitis E virus,amino acid sequence,Salmonella typhimurium,anti-HEVantibody
