摘要
【目的】探讨不同剂量电离辐射后大鼠类成骨细胞生物学性状的改变。【方法】成骨细胞通过酶消化法从SD大鼠乳鼠颅骨中获取传代 ;将第 2代细胞分别进行 0、1、4、6、9Gy的 γ线辐射 ,检测成骨细胞的增殖、碱性磷酸酶活性和骨钙素分泌量。【结果】细胞增殖、功能和分化受γ线电离辐射抑制的程度不同 ,1Gy时增殖即受到抑制 ,骨钙素分泌量在 4Gy时才受到显著地抑制 ,而碱性磷酸酶活性值甚至在 6Gy下抑制作用才有意义。【结论】电离辐射后 ,成骨细胞的增殖、功能和分化均受到抑制 ,但各指标对电离辐射的敏感性不同。
Objective To compare the effects of radiation of 5 different doses on the rat osteoblast-like cells (ROB) and to study the critical size of radiation, in which proliferation, physiological function and differentiation of the ROB were damaged. Methods The kind of ROB used in the present study was isolated from the calvariae of neonatal (one-day-old) SD rats by sequential enzymatic digestion. The third passage of the cell was used for all the experiments. Cells were irradiated with γ-ray from a 60 Co source at the experimental doses of 0, 1, 4, 6 and 9 Gy. The medium was changed immediately after irradiation. 6 days after radiation (9 days in culture), the relative cell number, alkaline phosphatase activity and the amount of bone Gla protein were measured. Results Radiation inhibited the ROB. However, the effects of radiation on the ROB differed from the three indicators. 1 Gy could restrain proliferation of the cells, and 4 Gy physiological function, but only when the dose was increased to 6 Gy was the effect on the differentiation significant. Conclusion The proliferation, physiological function and differentiation of the ROB are different in terms of their sensitivity to the ionizing radiation. The critical size of the radiation on ROB is uncertain, depending on the different indexes.
出处
《中山医科大学学报》
CSCD
北大核心
2002年第2期97-98,102,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省重点科技攻关基金资助项目 ( 96 2 2 0 44 0 5 )
