摘要
为观察模拟失重和辐射对细胞结构和功能的影响 ,用回转器模拟失重 ,60 Co -γ射线辐照体外培养的心肌和成骨细胞。结果表明 :( 1 )模拟失重时 ,心肌细胞的直径缩小近40 % ;对细胞骨架有明显的影响 ,微丝分布不均匀 ,无明显的方向性 ;对成骨细胞分裂、增殖活性有明显的影响 ,呈现分裂功能降低 ,增殖活性受抑制 ;( 2 ) 2Gy60 Co -γ射线辐照对培养心肌细胞超氧化物歧化酶 (SOD)、丙二醛 (MDA)含量及乳酸脱氢酶 (LDH)活性有较显著影响 ,特别是辐射和模拟失重共同作用时 ,则呈现一定的协同效应趋势。
The effects of simulated weightlessness and γ-irradiation on the structure and function of the myocardial cells and osteoblasts were investigated with a rotating clinostat and 2 Gy 60 Co γ-irradiation.The results showed:(1) Under the simulated weightlessness(SWL),the diameter of the myocardial cells decreased 40%.And the long-short diameter proportion decreased 70%,(P<0.01);SWL affected the cell skeleton and the distribution of microfilament became ununiform,the orientation was unclear;SWL had significant effects on osteoblast desintegration and proliferation.The desintegration function decreased and the proliferation process was inhibited;(2) 2 Gy 60 Co γ-ray irradiation had significant effects on Lactate Dehydrogenase (LDH) activity and Superoxide Dismutase( SOD),Malonaldehyde(MDA) content in the myocardial cells,(P<0.05);(5)A synergistic effect was observed between irradiation and SWL.
出处
《航天医学与医学工程》
CAS
CSCD
1998年第5期313-317,共5页
Space Medicine & Medical Engineering
关键词
模拟失重
辐照
细胞培养
心肌细胞
成骨细胞
simulated weightlessness irradiation cell culture myocardial cell osteoblast
