摘要
目的：探讨人类鼻粘膜上皮细胞应答组织缺氧，分泌合成血管内皮生长因子（ＶＥＧＦ）的能力，以及缺氧调节ＶＥＧＦ的可能机制。方法：将人类鼻粘膜上皮细胞分别在常氧（２１％）和缺氧（１％）条件下进行无血清原代细胞培养３～４８小时，另外常氧培养的其中一组加入金属镍共同孵育，采用原位杂交和酶联免疫吸附试验（ＥＬＩＳＡ）的方法检测各种条件下ＶＥＧＦ的ｍＲＮＡ水平和蛋白质水平。结果：① 缺氧条件下，ＶＥＧＦ的ｍＲＮＡ水平和蛋白质水平明显升高，与常氧组比较Ｐ＜０．００１，且具有时间依赖性；② 加入金属镍共同孵育，ＶＥＧＦ的表达也升高，与常氧组比较Ｐ＜０．０１。结论：在人类鼻粘膜上皮细胞中，缺氧可明显上调ＶＥＧＦ的ｍＲＮＡ和蛋白质表达，它的机制可能是通过含铁血红素氧敏感分子，一个与促红细胞生成素（ＥＰＯ）调节相似的途径。
Objective: To study the ability of response to tissue hypoxia and induce vascular endothelial growth factor(VEGF) in human nasal mucosal epithelial cells(HNMECs),the regulate mechanism of VEGF in hypoxia condition was investigated. Methods: HNMECs were cultured without serum under normal(21% O2)(group A) or hypoxia(1% O2)(group B) for 3～48% hours,and the third group was normal oxygen+n ickel for culture(group C). The levels of VEGFmRNA and VEGF protein were detected by assay of situ hybridixation and ELISA. Results:(1) The expression of VEGFmRNA and VEGF protein were increased significantly with time-dependence in group B,compare with control group(group A) showed P<0.01.(2) In group C,the expression of VEGFmRNA and VEGF protein were increased than that in group A(P <0.01),it showed that the nickel have ability of stimulate increase of VEGFmRNA and VEGF protein. Conclusions: Under hypoxia,the expressions of VEGFmRNA and VEGF protein appeared up-regulates in HNMECs. Our experimental result showed that the induction of VEGFmRNA and VEGF protein expression by HNMECs in response to hypoxia culture condition should be modulated by an oxygen-sensing hemelike protein similar way to regulation of the erythropietin gene. [Chinese Arch Otolaryngol Head Neck Surg,2002;9(1):46～49 From the Department of Otolaryngology-Head and Neck Surgery,China-Japan Union Hopital of Jilin University,Changchun 130031(Dr. Jiang Shu)]
出处
《耳鼻咽喉（头颈外科）》
2002年第1期46-49,共4页
Chinese Arch Otolaryngology-Head Neck Surg
