摘要
目的 :检测鼻息肉组织和鼾症下鼻甲粘膜组织中的血管内皮生长因子 (VEGF) m RN A水平的表达 ,了解其在慢性炎症过程中的作用。方法 :取 6例行下鼻甲切除术的下鼻甲粘膜和 7例鼻息肉切除术的鼻息肉标本 ,用半定量的反转录 -聚合酶链反应 (RT- PCR)方法检测 VEGF的 m RNA表达。结果 :RT- PCR结果显示在鼻息肉组织中 V EGF的表达较鼾症患者下鼻甲粘膜组织明显升高。结论 :鼻息肉组织中 VEGF的表达显著升高 ,推测 VEGF在鼻息肉的形成、生长及复发过程中具有极其重要的作用。
Objective:To explore the pathogenesis of nasal polyps,the aim of this study is to detect the expression of vascular endothelial growth factor (VEGF) in nasal polyp(NP) tissue, and compare these findings with normal nasal turbinate.Method:We examined the expression of VEGF in human nasal polyps from patients underwent nasal polypectomy (n=7) and nasal turbinate from patients with snoring (n=6). VEGF mRNA expression was determinded by semi quantitative reverse transcription polymerase chain reaction (RT PCR).Result:VEGF mRNA expression was significantly stronger in the eight NP than in the turbinate tissue growp (P< 0.05 ). Conclusion:We conclude that VEGF expression is upregulated in NP,which suggests a potential role of VEGF in the formation and growth and recrudescence of NP.
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2001年第8期339-340,343,共3页
Journal of Clinical Otorhinolaryngology
关键词
鼻息肉
血管内皮生长因子
聚合酶链反应
Nasal polyp
Vascular endothelial growth factor
Polymerase chain reaction
