摘要
选用 1170只 2 11日龄中华鳖进行养殖试验 ,研究不同剂量 (0 ,5 0 ,10 0 mg/kg)的 l-肉碱对中华鳖脂肪代谢的影响 .通过解剖取样测定肝脏、四肢肌肉和脂肪块的脂肪代谢酶活性及肌细胞内脂肪酸β-氧化反应等生化指标 ,探讨其作用机理 .结果表明 ,在饲料中添加 10 0 mg/kg l-肉碱显著降低了中华鳖四肢的脂肪沉积量的 2 7.11% (P<0 .0 1) ,肝脏和脂肪中的苹果酸脱氢酶及脂肪中激素敏感脂酶总活力分别提高了 2 4 .32 % (P<0 .0 1)、32 .2 2 % (P<0 .0 1)和 14 .88% (P<0 .0 5 ) ,肌肉和脂肪中脂蛋白脂酶总活力分别提高了 2 7.0 8% (P<0 .0 1)和 13.18% (P<0 .0 5 ) ,肌肉中游离肉碱、总酸不溶肉碱含量分别升高了 16.92 % (P<0 .0 1)和 38.2 4 % (P<0 .0 1)
One thousand one hundred and seventy Chinese soft shelled turtles allotted to three groups by weight and sex were conducted to study the effects of carnitine on adipose metabolism Every group included three replicates of one hundred and thirty turtles each The groups received the same basal diet supplemented with 0, 50, 100 l carnitine respectively during the period of 211 to 360 day ages The results showed that 100 mg/kg l carnitine treated group was the most effective, which decreased the weight of fat deposited in extremities by 27 11% ( P <0 01) Data from samples analyses indicated that adding 100 mg/kg l carnitine significantly increased activity of malic dihydrogenase of liver and fat by 24 32% ( P <0 01)and 32 22% ( P < 0 01), and that of hormone sensitive lipase of fat by 14 88% ( P <0 05),and that of lipoprotein lipase of muscle and fat by 27 08% ( P <0 01)and 13 18% ( P <0 05), and the content of free carnitine and total acid insoluble cartinine of muscle by 16 92% ( P <0 01)and 38 24% ( P <0 01)
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2002年第1期70-73,共4页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省科技厅"九五"重点攻关项目 ( 962 10 2 0 76)
关键词
中华鳖
肉碱
脂肪代谢
脂蛋白酯酶
脂肪酸氧化
饲料
Chinese soft shelled turtle
carnitine
adipose metabolism
lipoprotein lipase
oxidation of fatty acid
