摘要
以水稻为模式植物研究了利用碱处理叶片直接用作 PCR扩增的模板 ,并应用于水稻品种种性和纯度鉴定以及分子标记辅助田间育种材料的选择中。该方法具有快速、简便、结果可信度高 ,重复性好 ,对待测植株的损伤小等特点 ,它无需高速冷冻离心机及相应的提取 DNA的试剂 ,故试验成本低 ,检测所需的时间短。尤其是群体较大时 ,此种方法更是显得经济有效 ,利用此种方法在 1 d内可检测 30 0份样品。
A new method for PCR reaction from a small quantity of rice leaf tissue with alkali-treated was presented, which can be applied either to identify genotype of rice variety and its purity, or to assist selection for molecular breeding. This method is rapid, simple and reliable, and only needs a small part of rice leaf tissue that does not damage the whole plant. It does not need high speed refrigerated centrifuge and the reagents for DNA extraction. Especially, it is more economic and effective for a large-scale PCR tests. Normally 300 samples can be processed within a day by this method.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2002年第1期67-70,共4页
Chinese Journal of Rice Science
