摘要
本文采用HPLC分析RU 486及其代谢产物。分析用200mm×3.5mm ODS/YWG柱(青岛海洋化工厂担体),流动相为甲醇:乙腈:水42:28:30(V:V:V),pH4.7,流速1ml/min,紫外检测为302nm。在此色谱条件下,能使RU 486及其三种代谢产物得到很好分离。四种化合物的标准曲线在100～2800ng/ml浓度范围内,线性的相关系数为0.999。RU 486和其代谢产物去单甲基衍生物(RU 42633),丙炔醇衍生物(RU 42698)不同浓度的回收率均达90%左右,另一代谢产物去双甲基衍生物(RU 42848)的回收率为60%左右,本方法已用于RU 486及其代谢产物的临床药代动力学研究。
A HPLC method has been developed for analysis of RU 486 and its metabolites Ana-
lyses were performed on 200 mm×3.5 mm ODS/YWG column. The mobile phase was
methanol: acetonitrile: water 42: 28: 30 (v: v: v), pH 4.7 and the flow rate was 1 ml/min.
The detection was monitored by UV detector at 302 nm. Under these chromatographic con-
ditions, RU 486 and its metabolites were well separated. The standard curves of these com-
pounds were linear over the range from 100 to 2800 ng/ml, with regression coefficient of
0.999. The recovery of different concentrations of RU 486, RU 42633 and RU 42698
were 90% and that of RU 42848 was 60%. This method has been applied to the study
of the pharmacokinetics of RU 486 and its metabolites.
出处
《生殖与避孕》
CAS
CSCD
北大核心
1991年第1期26-31,共6页
Reproduction and Contraception
