摘要
本实验以二甲基亚砜(Dimethyl Sulfoxide,DMSO)为诱导剂,诱导人早幼粒白血病细胞系HL-60沿粒系统成熟分化。动态观察了诱导后1—6天HL-60的形态、功能成熟度改变、膜流动性和增殖活性的变化。结果表明,早幼粒细胞由诱导前的78％降至5％,杆核与分叶核细胞由6％和1.5％分别增加至32.5％和5.5％。NBT(Nitroblue tetrazolium,四唑氮蓝)还原试验显示其功能亦渐超成熟。诱导后HL-60 DNA合成速率明显降低(降低40～75％),膜脂流动度亦显著降低(降低30％,P<0.01)。提示诱导分化后的HL-60细胞不仅获得了与正常成熟粒细胞相似的形态功能特征,同时还失去了膜流动性增高及快速繁殖增生的恶性特征。
By using DMSO as an inducer, we observed the change of membrane fluidity and DNA synthesis rate of HL-60 cells which were undergoing differentiation in six successive days. The results showed that promyelocyte was decreased from 78% before differentiation to 5% after differentiation, and stabnuclear granulocyte and segmented neutrophils were increased from 4% and 1.5% to 32.5% and 5.5% respectively. NBT reduction test showed functional maturity of the differentiated HL-60 cells. DNA synthesis rate and membrane fluidity decreased obviously (deceased 40-75% and 30% respectively). Our results suggested that induced differentiated HL-60 cells not only aquired morphologic and functional characteristics of normal mature granulocyte but also lost the malignent characteristics of increased membrane fluidity and high speed of proliferation.
关键词
白血病
细胞
分化
膜流动性
DMSO
HL-60 Cells
DMSO
Induced differentiation
Membrane fluidity
