摘要
目的利用自旋标记—顺磁共振技术研究严重烧伤大鼠早期心肌细胞膜的变化。方法用5-氮氧自由基硬脂酸(5-doxylstearic acid,5NS)和16-氮氧自由基硬酯酸((16-doxylstearic acid,16NS)分别掺入到不同深度的膜类脂区,观察严重烧伤对大鼠心肌细胞不同深度的膜类脂区流动性的影响,同时用3-甲基马来酰亚胺氮氧自由基(3-maleidoproxy1,3MP)标记膜蛋白巯基,观察膜蛋白旋转相关时间(τ_c)及强固定化与弱固定化组分谱线高度比值(h_s/h_w)的变化。结果严重烧伤大鼠早期心肌细胞膜脂浅层的序参数(S)值无明显变化,而膜脂深层 S 值增大,膜蛋白 h_s/h_w 显著增大,膜蛋白旋转相关时间τ。也显著增大。结论严重烧伤后早期即出现心肌细胞膜的损伤,表现为膜脂质深层流动性下降,膜蛋白构象改变,膜蛋白运动受限。这些变化可以直接影响细胞膜蛋白的功能,从而导致心肌细胞功能的变化。
Objective To demonstrate the mechanism of early cardiac myocyte membrane damage at molecular level in severely burned rats.Methods SD rats were randomly divided into sham-burn and burn groups.A 30% TBSA burn was created in burn group.Hearts were obtained at different time post- burn.Cardiac myocytes isolated by collagenase using Langendoff perfusion apparatus were spin labeled with 5-doxylstearic acid(5NS),16-doxylstearie acid(16NS)and 3-maleidoproxy1(3MP).The order parame- ters(S value),h_s/h_w ratio and rotational correlation tim
基金
本课题受国家自然科学基金资助
