摘要
〔目的〕建立Hplc法测定肉与肉制品中雌二醇、已烯雌酚、沙丁胺醇残留量的方法。〔方法〕样品经甲醇提取 ,提取液浓缩后经 0 .45 μmFH滤膜过滤 ,进高效液相色谱分析。〔结果〕得到雌二醇、已烯雌酚、沙丁胺醇浓度与峰面积成正比的工作曲线 ,相关系数r>0 .999;其相对标准差RSD≤ 0 .6 % (n =6 ) ,检出限≤ 2ng,回收率范围为 95 .8～ 10 2 .5 %。〔结论〕该法具有灵敏、准确、精密、样品处理简单。
睴bjective〕To study a method for the determination of estradiol, diethylstilbestrol and salbutamol residue in meat and meat produce by HPLC.〔Method〕 Estradiol, diethylstilbestrol and salbutamol were extracted from homogenized sample with methanol, then the HPLC method was used to analyze estradiol, diethylstilbestrol and salbutamol residue in meat and meat produce.〔Results〕 Good separation was achieved in the 3 kinds component and the standard curve showing a direct ratio between the concentration and the peak area were obtained, r>0.999,RSD≤0.6%(n=6);the detection limits were below 2ng; The recovery percent were between 95.8% and 102.5%. 〔Conclusion〕 The method proved to be satisfactory in precision, sccuracy and sensitivity. The preparation of sample was simple.
出处
《中国卫生检验杂志》
CAS
2001年第6期654-655,共2页
Chinese Journal of Health Laboratory Technology
