摘要
目的 研究胃及十二指肠液食管反流对食管内抑癌基因、癌基因表达的影响及与黏膜损伤的关系。方法 制作单纯胃食管反流 (G组 )、单纯十二指肠食管反流 (D组 )、十二指肠胃混合食管反流 (DG组 )及无反流对照组 (C组 )动物模型 ,用免疫组化法 (SABC)检测各组不同时期食管上皮 p5 3、细胞周期素D1(CyclinD1)、p2 1、p16等基因的表达。 结果 反流组食管组织中 p5 3、CyclinD1基因表达均显著高于C组 ,并随病程延长而明显增强 ,D组表达又强于G组 ;各组不同时期p16表达无明显差异 ;p2 1表达在D组、DG组较C组为低 ,且与 p5 3蛋白表达呈负相关。 结论 食管内胃及十二指肠反流物均可改变食管上皮p5 3、CyclinD1、p2 1基因的表达 ,十二指肠内容物的作用更明显。但反流对 p16基因的表达影响不大。p5 3、CyclinD1、p2 1等癌基因或抑癌基因的表达改变可能参与反流性食管炎及其并发症的发生。
Objective To investigate the effects of gastroesophageal reflux and duodenoesophageal reflux on expression of p53, Cyclin D 1, p21 and p16 in esophageal epithelium in rats. Methods Rat models of gastroesophageal reflux (G), duodenogastroesophageal reflux (DG), duodenoesophageal reflux (D) and control group (C) were set up by operations. All esophagi were draw at 1,2,4 week after operation and the expressions of p53, Cyclin D 1, p21 and p16 protein were measured by immunohistochemistry analyses. Results The levels of p53, Cyclin D 1 protein expression in reflux groups were significantly greater than those in C group, and those in D and DG group were significantly higher than in G group. The expressions of p16 protein were similar in each group. The number of p21 positive cells in D and DG group was fewer than C group at 4 week, and the staining pattern correlated well with p53 staining. Conclusions Both gastric juice and duodenal contents can change expression of p53, Cyclin D 1, p21 protein in esophageal epithelium, but not that of p16. The effects of duodenal contents is stronger than gastric juice. The changes of above genes expression may associate with the pathogenesis of reflux esophagitis and its complications.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2001年第10期587-589,共3页
Chinese Journal of Digestion
基金
上海市卫生系统百人计划基金资助项目 ( 98BR0 2 6)
