摘要
采用SD大鼠进行不同手术 ,制作单纯胃食管反流组 (G组 )、单纯十二指肠食管反流组 (D组 )、胃十二指肠混合液食管反流组 (DG组 )及无反流对照组 (C组 )模型 ,于术后分批取得食管标本 ,制备单细胞悬液、经DNA染色后行流式细胞仪检测。分析样品的DNA指数 (DI)、增值指数 (PI)及非整倍体率。结果发现 ,1周时各组食管上皮的平均PI均无明显差别 ;第 2周D组平均PI增多 ,与C组相比有显著差异 (P <0 0 5 ) ,但各反流组之间以及DG、G组与C组之间均无明显差别 ;第 4周D组、DG组的平均PI显著增加 ,明显高于G组和C组 ,后 2组间差别无统计学意义。三个时间点各组食管上皮的平均DI相差均不显著 ,所有样品均未见非整倍体的存在。说明 3种反流均可致食管上皮细胞增殖增加 ,有十二指肠内容物反流的组此表现更为明显。提示十二指肠内容物反流对食管的损伤作用较强 。
Gastroesophageal reflux(G), duodenoesophageal reflux(D) and duodeno gastroesophageal reflux(DG) model and control group(C) were reproduced by operations in rats. Their esophagi were harvested at 1, 2, and 4 weeks after operation. Epithelial cells were stained by PI and assessed with flow cytometry(FCM).The results of FCM were compared in terms of DNA index (DI, the ratio of the G0/G1 peak of the sample cells to that of the spleen lymphocyte of normal rat), proliferating index (PI, the percent of cells in S and G 2 /M in all cells) and aneuploidy (a distinct additional G0/G1peak).PI of D group increased at 2 weeks, and PIs of D and DG group were greater than G group and C group at 4 weeks.DIs and the rates of aneuploidy of all groups were similar at all time points. The results were in accord with the morphological changes. It is concluded that gastric and duodenal contents can induce abnormal proliferation of esophageal epithelium. This effect is obvious in groups with reflux of duodenal contents. Excessive proliferation of the esophageal epithelium might be one of the pathogenetic factors of reflux esophagitis or other relevant complications.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2001年第10期715-717,共3页
Medical Journal of Chinese People's Liberation Army
基金
上海市卫生系统百人计划基金资助课题 (编号 98BR0 2 6 )
