摘要
目的 :观察颈淋巴结靶向性葫芦素BE聚乳酸纳米微粒 (CuBE_PLA_NP)冻干针剂的急性毒性作用和对局部组织的刺激反应 ,为动物实验和临床实验提供依据。方法 :用不同剂量的CuBE_PLA_NP冻干针剂和葫芦素BE(CuBE)行小鼠皮下和尾静脉注射 ,观察小鼠用药后的毒副反应 ,并计算半数致死量 (LD50 ) ;用CuBE_PLA_NP冻干针剂和CuBE行兔股四头肌注射 ,观察药物对局部组织的刺激性。结果 :CuBE_PLA_NP冻干针剂皮下和静脉注射的LD50 值分别为 16 74mg/kg± 1 83mg/kg和 5 6 0mg/kg± 0 83mg/kg,CuBE皮下和静脉注射的LD50 值分别为 8 6 3mg/kg±1 0 0mg/kg和 2 5 0mg/kg± 0 30mg/kg。CuBE_PLA_NP冻干针剂局部肌肉注射的反应数均为 0级 ,CuBE的反应数为0～ 1级。结论 :CuBE_PLA_NP冻干针剂对机体的毒副作用和局部刺激性小于CuBE 。
Objective: The aim of this experiment was to study acute toxicity and local stimulation of cucurbitacinBE poly_lactic acid nano_particles (CuBE_PLA_NP) for targeting cervical lymph node. Methods: Various doses of CuBE_PLA_NP lyophilization injection and cucurbitacinBE (CuBE) were hypodermically or veno_injected into the mice, and then the drug toxicity and side effects were observed; 50% lethal dose (LD\-\{50\}) was counted. CuBE_PLA_NP lyophilization injection and CuBE were also injected into the quadriceps femoralis muscle of rabbits to observe the local stimulate responses of drugs.Results: The fifty percent lethal doses of subcutaneous and venous CuBE_PLA_NP in mice were 16 74 mg/kg±1 83 mg/kg and 5 60 mg/kg±0 83 mg/kg respectively, that were higher than those of CuBE which were 8 63 mg/kg±1 00 mg/kg and 2 50 mg/kg±0 30 mg/kg respectively. Local stimulate response in rabbit quadriceps femoralis muscle after CuBE_PLA_NP and CuBE injected are 0 grade and 0 1 grade respectively. Conclusion: The acute toxicity of CuBE_PLA_NP is lower than that of CuBE. Both CuBE_PLA_NP and CuBE are adequate for local injection, but the local stimulation of CuBE_PLA_NP is smaller than that of CuBE.\;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2001年第6期380-382,共3页
West China Journal of Stomatology
基金
卫生部科学基金资助项目 (编号 98_1_2 2 1)
