摘要
【目的】建立人前脂肪细胞原代培养方法 ,以更深入地研究人体脂肪组织增生的生物学特征。【方法】选用成人的腹部脂肪组织 ,采用原代消化细胞培养法培养出棱形细胞 ;同时取皮肤组织 ,进行成纤维细胞培养作为对照。【结果】培养出的棱形细胞成分均一 ,增殖旺盛 ,分化率高。经形态学动态变化的观察 ,生长曲线及油红O脂肪染色抽取法测定 ,证明是功能活跃的前脂肪细胞 ,并在体外重现了其增殖的全过程。【结论】在成熟的脂肪组织中存在着可分化成熟、生成脂肪的前脂肪细胞。由于脂肪细胞是胰岛素作用的经典靶细胞之一 ,本实验为进一步研究与肥胖及胰岛素抵抗相关的疾病如多囊卵巢综合症等打下了基础。
To establish a primary human preadipocyte culture method for better understanding the properties of hyperplasia of human adipose tissue. Using primary cell culture, fibroblast like cells from adult abdominal adipose tissue were cultured. Fibroblasts from adult human dermis were also cultured to serve as control. The cells were highly homogeneous, proliferative and had a high differentiation rate. Their dynamic morphological changes, growth curve, extracting stained intracytoplasmic lipid with oil red O, all verified their preadipocyte identity. Under controlled conditions, the preadipocytes replayed their hyperplasia process in vitro.[Conclusion] In mature human adipose tissue, there exist preadipocytes that can different into mature adipocytes. Since adipocyte is one kind of classic target cells to insulin, this study laid the basis for further probing into obesity and insulin resistant diseases such as polycystic ovary syndrome.
出处
《中山医科大学学报》
CSCD
北大核心
2001年第6期443-446,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
广东省卫生厅科研基金资助课题 ( 2 0 0 1189)
