摘要
目的更深入地研究人体脂肪组织增生和肥大的生物学特征。方法选用成人的纯脂肪颗粒,采用“贴壁-天花板”培养法培养出梭形细胞。结果培养出的梭形细胞成分均一,增殖旺盛,分化率高。经形态学动态变化、生长曲线、甘油磷酸脱氢酶活性变化、油红 O 脂肪染色及对胰岛素和地塞米松反应的测定,证明是功能活跃的前脂肪细胞,并在体外重现了其增殖、肥大的全过程。结论在成熟的脂肪组织中存在着功能活跃的可调控组分。这是在国内首先建立的一种新的简便的培养人前脂肪细胞的方法,为进一步研究肥胖及探讨提高脂肪移植成活率奠定了扎实的基础。
Objective To better understand hyperplasia and hypertrophy properties of human adi- pose tissue.Methods Using an“adhesion-ceiling”culture strategy,fibroblast-like cells from adult pure adi- pose granules were successfully cultured.Results The cells were highly homogeneous,highly proliferative and with a high differentiation rate.Their dynamic morphological changes,growth curve,glycerophosphate dehydrogenase pattern,oil red O staining,and reaction to insulin and dexamethasone all verified their preadipocyte identity.Under controlled conditions,the preadipocytes replayed their in vivo hyperplasia and hypertrophy process.Conclusion In mature human adipose tissue there exist functionally active fractions that can be modified.This study laid the basis for further probing into obesity and fat autotransplantation.
基金
本课题受上海市科学技术发展基金资助(编号:94411203)
