摘要
对血管内皮细胞体外培养的常规方法进行了改进 ,采用胰蛋白酶 胶原酶 (1∶1)混合酶液消化方式分离人脐静脉内皮细胞 ,简化完全培养液的组成成分 (不添加血管内皮细胞生长因子、肝素等辅助因子 ) ,机械刮擦法分离单层细胞进行传代培养。培养细胞用形态学、超微形态学和免疫组织化学染色法进行鉴定 ,证实培养的细胞是血管内皮细胞。
In order to improve the routine culture method for vascular endothelial cells, endothelial cells of vein from newborn umbilical cord were successfully isolated with combined using 0 1% collagenase and 0 25% trypsin (1∶1). The endothelial cells were further abrated by mechanical method. The composition of culture medium was simplified by not adding vascular endothelial cell growth factor and heparin. The cultured cells were verified as vascular endothelial cells by morphology, ultramicromorphology and immunohistochemistry.
出处
《卫生研究》
CAS
CSCD
北大核心
2001年第3期188-189,F004,共3页
Journal of Hygiene Research
关键词
人脐静脉
内皮细胞
细胞培养
鉴定
human umbilical vein, endothelial cells, cell culture
