摘要
于1987年,用盐度为0,100,200,500,1000mmol/L NaCl的Hoagland完全培养液培养碱蓬,测定幼苗地上部分膜脂过氧化产物丙二醛(MDA),同时研究超氧化物歧化酶(SOD)的活性。结果表明,(1)盐胁迫明显影响MDA含量:12h之内,各种盐胁迫均使MDA量较对照组低;12h之后,低盐度(100mmol/L)继续使MDA较对照降低,而高盐度(≥200mmol/L)则促使MDA累积。(2)碱蓬体内SOD活性也明显受到盐胁迫的影响:12h之内,不同盐胁迫均使其活性较对照增大;12h之后,低盐度继续增加SOD活性,而高盐度则降低其活性。MDA和SOD活性之间存在负相关性。上述结果表明,碱蓬的盐伤害与自由基有关。
Seepweed, a halophytic plant, were collected from marine marshes in Dongying city and cultured in greenhouse from seed germination to seedling in 1987. The seedlings as experimental materials were treated with Hoagland nutrient solution containing 0,100,200,500,1 000 mmol/ L NaCl. The changes of membrane-lipid peroxidation after salt treatment were analysed by determining malondialdehyde (MDA). We also studied the reason of membrane-lipid peroxidation by meansuring superoxide dismutase (SOD) activity.The results showed that MDA and SOD activity were related to salinity. A marked decrease of MDA and significant increase of SOD activity were observed within 12 h salt trea+-ment. After 12h, lower salinity (100mmol/L NaCl) led a decrease of MDA and an increase of SOD activity, but high salinity (≥200 mmol/L NaCl) led to an accumulation of MDA and decrease of SOD activity. There was a negative correlationship between MDA and SOD activity.The salt injury of seepweed was partially resulted from membrane lipid-peroxidation induced, by free radicals.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1991年第6期579-582,共4页
Oceanologia Et Limnologia Sinica
