摘要
目的 探讨IFN γ、IL 2或B7 1基因修饰对胆管癌细胞免疫原性的作用。方法 构建携带人IFN γ或IL 2基因的逆转录病毒表达载体 ,和含人B7 1基因的腺病毒载体 ,导入胆管癌细胞系QBC939,运用淋巴细胞杀伤试验、肿瘤细胞在裸鼠体内成瘤性分析、混合淋巴细胞和肿瘤细胞培养 ,研究导入的目的基因对QBC939细胞免疫原性的影响。结果 导入IFN γ基因的QBC939细胞体外能增强胆管癌病人外周血淋巴细胞杀伤亲本瘤细胞的活性 ;导入IL 2基因的QBC939细胞在裸鼠体内的成瘤性降低 ;导入B7 1基因的QBC939细胞能刺激外周血T淋巴细胞的增殖。结论 人IFN γ、IL 2或B7
Objective To investigate the change of immunogenicity of cholangiocarcinoma cell line QBC939 modified by human interferon γ, IL 2 or B7 1 genes. Methods The retroviral vector of IL 2 or IFN γ cDNA, and adenoviral vector containing B7 1 cDNA were constructed and transduced into QBC939 cell line. Cytotoxicity lymphocyte assay,tumorigenicity of tumor cell in nude mice, and mixed lymphocyte and tumor cell coculture were employed to study the change of immunogenicity of the cholangiocarcinoma cell line QBC939 transduced with interferon γ, IL 2, or B7 1 gene. Results The cytolytic activity of cholangiocarcinoma patient's peripheral blood lymphocytes against parental cells could be enhanced by in vitro QBC939 after modification with IFN γ gene. The tumorigenicity of QBC939 cell transduced with IL 2 gene could be reduced in nude mice. QBC939 tranduced with B7 1 could stimulate the proliferation of human peripheral blood T lymphocytes. Conclusion The immunogenicity of QBC939 cell lines could be enhanced by human interferon γ, IL 2, or B7 1 cDNA.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2002年第2期146-148,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 (3 0 0 0 0 0 77)
