摘要
目的：研究腺病毒介导的多基因（GM－CSF、B7-1、p53、IL－2）在胆管瘤细胞系QBC939中的表达及对其生长的影响。方法：构建同时含GM－CSF、B7－1、p53、IL－24种目的基因的重组腺病毒载体Admultigenes，应用流式细胞仪、ELISA、免疫织化等方法，分析该重组腺病毒体包含的目的基因在靶细胞中的表达，并绘制细胞的生长曲线。结果：腺病毒介导的多基因在胆管癌细胞系QBC939中高效表达（IL－2不表达），并能抑制胆管瘤细胞的生长。结论：腺病毒介导的多种基因能在胆管瘤细胞QBC939中表达．并抑制肿瘤细胞的生长。
Objective: To study the expression rate of adenovirus-mediated human genes of GM-CSF,B7-1, p53 and IL-2 in the cells of cholangiocarcinoma cell line QBC939 and their effects on the growth of the malignant cells. Methods: Recombinant adenovirus was constructed to contain human genes of GM-CSF,B7-1, p53 and IL-2 and the expression rate of the genes in the ce1ls of hurnan cholangiocarcinoma celI line QBC939 was detected with ELISA, immunohistochemistry and FACS. In addition, the growth of the malignant cells was deIineated. ResuIts: Adenovirus-mediated multigenes except IL-2 were expressed in high rate in the malignant cells and they inhibited the growth of the cells. Conclusion: Adenovirus-mediated human genes of GM-CSF, B7-1 and p53 can be expressed in cholangiocarcinoma cell line QBC939 and inhibit the growth of the cells.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第10期742-745,共4页
Journal of Third Military Medical University
关键词
多基因腺病毒
胆管癌
基因表达
癌细胞
GM-CSF
B7-1
p53
IL-2
cholangiocarcinoma cell line
gene expression
growth inhibition
