摘要
基于苯胺被Ｈ２Ｏ２氧化为硝基苯使其荧光猝灭的原理建立了一种测定中药对Ｈ２Ｏ２清除率的新方法。研究表明：苯胺荧光在ｐＨ７～８之间有最大强度；乙醇浓度为５０％（Ｖ／Ｖ）时，瑞利散射干扰最小．荧光强度最大；苯胺浓度为１１０ ｍｍｏｌ／Ｌ，Ｈ２Ｏ２的浓度小于１ｍｍｏｌ／Ｌ时，苯胺荧光猝灭程度与Ｈ２Ｏ２浓度呈线性关系。测定了中药厚朴和杜仲对Ｈ２Ｏ２＝的清除率，结果与鲁米诺化学发光法测定值一致。
Based on principle that aniline can be oxidized as nitrobenzene and its fluorescence quenched by H2O2, a new method is developed to determine eliminating ratio of Chinese traditional medicines for H2O2. It is shown that fluorescence of aniline is maximum when pH value is between 7 and 8. Rayleigh scattering interference is minimum and fluorescence is maximum when the concentration of ethanol in sample solution is 50% (V/V). Linear relation between the quenching level of aniline fluorescence and the concentration of H2O2 is established when the concentration of H2O2 is below 1 mmol/L. Eliminating ratios of Cortex Magnoliae officinalis and Cortex Eucommia for H2O2 are determined. The results are identical with those of luminol chemiluminescence.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2002年第2期183-185,共3页
Chinese Journal of Analytical Chemistry
基金
河北省自然科学基金(No.202281)
河北省教育厅自然科学基金(No.2000257)
