摘要
目的:探讨益骨口服液含药血清对成骨细胞增殖、分化及矿化功能的影响。方法:取SD大鼠40只,雌雄各半。将大鼠分为A组(高剂量组)、B组(中剂量组)、C组(低剂量组)、D组(空白组),E组(对照组)。按照不同的剂量灌胃。灌胃10d后,制备含药血清;将从乳鼠颅骨取材的成骨细胞培养于含药血清培养液中,然后应用MTT法、硝基苯磷酸盐法及茜素红染色方法观察体外培养成骨细胞、碱性磷酸酶及矿化结节的变化。结果:经过10d益骨口服液灌胃后采血制备的含药血清明显增加了成骨细胞数量(P<0.05或P<0.01)、碱性磷酸酶的表达水平及矿化结节的形成明显。结论:证实了益骨口服液含药血清可以促进成骨细胞增殖、分化及矿化。
Objective：To investigate the effects of serum containing Yigu oral liquid（SYOL）on the proliferation,differentiation and mineralization of the osteoblasts.Methods：Forty SD rats were randomly divided into 5 groups：group A（high dosage group）,group B（midle dosage group）,group C（low dosage group）,group D（blank group）,and group E（control group）.Each group was given different dosage of SYOL with intragastric administration.Ten days later,all the rats were sacrificed to collect SYOL.Meanwhile,the osteoblasts were obtained from the skull of neonate rat and cultured in SYOL.MTT assay was used to observe the proliferation of osteoblast.ρ-nitrophenylphosphat was used to measure the ALP of osteoblast.The alizarin bordeaux staining was performed to detect the mineralization nodal.Results：SYOL remarkably increased the quantity of osteoblast（P0.05 or P0.01）,the level of the ALP expression and the formation of mineralization nodal.Conclusion：SYOL can promote the proliferation,differentiation and mineralization of osteoblast.
出处
《中国中医骨伤科杂志》
CAS
2010年第1期6-8,共3页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
浙江省科技厅基金资助项目
编号:2007C33019
