摘要
目的:探讨组织型纤溶酶原激活剂（tissue-type plasminogen activator,tPA）对人视网膜色素上皮（retinal pigment epithelial,RPE）细胞的作用。方法:运用活细胞计数法和甲基噻唑基四唑（methyl thiazolyl tetrazolium,MTT）比色实验观察分析tPA对人 RPE细胞增殖的抑制作用,应用流式细胞仪（flow cytometry,FCM）分析人RPE细胞周期。结果:0.1～3μg/mltPA对人RPE细胞有抑增殖作用,其效应与药物浓度和作用时间呈正向依赖关系（P<0.01）;5μg/ml tpA对人 RPE细胞有致死毒性作用。FCM显示 tPA作用后 S期细胞增加9.8％（P<0.05）,G2M期减少6.6％（P<0.05）。结论:tPA在一定时间和剂量范围内对人RPE细胞具有抑增殖作用,且不对细胞产生致死毒性;tPA主要延迟/阻滞人RPE细胞于S期。眼科学报2001;17:122～125。
Purpose: To study the inhibition effects of human retinal pigment epithelial (RPE) cells by tissue-type plasminogen activator (tPA).Methods: The effects of tPA on human RPE cells proliferation were studied by viable cell counting and methyl thiazolyl tetrazolium (MTT) colorimetric assay. Flow cytometry (FCM) analysis was used to examine RPE cell cycles.Results: The proliferation of human RPE cells could be inhibited by 0. 1~3μg/ml tPA(P<0. 05). The effects of inhibition were depended on the drug density and the action time directly ( P<0.01). 5μg/ml tPA was deadly-toxic to human RPE cells. FCM revealed that the cells in S phase increased 9. 8% (P <0.05) and cells in GaM phase decreased 6. 6% (P<0. 05), compared to controls.Conclusion: tPA at a certain dose rang and period could inhibit human RPE cells proliferation without deadly-toxicity. The human RPE cells growth could be interfered in S phase by tPA. Eye Science 2001; 17: 122 - 725.
出处
《眼科学报》
2001年第2期122-125,共4页
Eye Science
关键词
组织型纤溶酶原激活剂
增殖
人视网膜色素上皮细胞
细胞周期
tissue-type plasminogen activator, proliferation, human retinal pigment epithelial cell, cell cycle
