摘要
目的 探讨多种生长调控因子在良性前列腺增生症 (BPH)中的发病机制。 方法 采用免疫组织化学SP法检测 15种细胞增殖和凋亡的生长调控因子 ,结合计算机图像分析处理系统进行定量测定 ;同时对BPH的组织构成中间质、上皮和腺腔各因子所占面积百分比进行图像定量分析测定。 结果 碱性成纤维细胞生长因子 (bFGF)、转化生长因子 β(TGFβ)、纤维连结蛋白 (FN)和Ⅳ型胶原表达与BPH的间质增生之间比较差别有显著性意义 (r =0 .32 3、0 .2 78、0 .2 35、0 .2 41,P <0 .0 5 ) ;而表皮生长因子受体 (EGFR)、胰岛素样生长因子 Ⅰ、神经生长因子 (NGF)以及凋亡诱导因子bax和ICE表达与BPH的上皮增生之间比较差别有显著性意义 (r =0 .315、0 .2 77、0 .2 46、- 0 .2 94、- 0 .2 5 2 ,P <0 .0 5 )。 结论 BPH的发生、发展与多种生长调控因子异常有关 ,其中bFGF是间质增生的主要作用因子 ,EGFR和bax是上皮增生的主要作用因子。
Objective To investigate the role of 15 kinds of growth regulatory factors in the pathogenesis of benign prostatic hyperplasia (BPH). Methods 15 kinds of growth regulatory factors involving cell proliferation and apoptosis were studied by immunohistochemistry with computer assisted quantitative image analysis.The mean percent area densities of stroma,epithelium and glandular lumen in BPH were examined by quantitative image analysis. Results Positive relations were found bewteen expressions of basic fibroblast growth factor (bFGF),transforming growth factor β, fibronectin, type Ⅳ collagen and proportions of stroma (r=0.323?0.278?0.235?0.241,P<0.05).Expressions of epidermal growth factor receptor (EGFR),insulin-like growth factor,nerve growth factor,bax and ICE significantly correlated with proportion of epithelia (r=0.315?0.277?0.246?-0.294?-0.252,P<0.05). Conclusions Abnormality of mutiple growth regulatory factors are involved in the pathogenesis of BPH,of which bFGF plays the most important role in promoting stromal hyperplasia while EGFR and bax being the much more important in promoting epithelial hyperplasia.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2001年第9期520-522,共3页
Chinese Journal of Urology
基金
南京军区医药卫生科研基金资助 ( 99No2 4)
