摘要
用间接ELISA法检测前列腺素E_1(PGE_1)对小鼠血清抗体形成的影响;用台盼蓝排斥试验检测NK细胞活性;用相差显微镜及透射电镜观察PGE_1作用的小鼠脾NK细胞杀伤S_(180)肿瘤细胞的形态及超微结构。结果表明PGE_1浓度在0.01μmol/L时可抑制小鼠血清抗体生成,抑制脾NK细胞活性,由PGE_1作用的NK细胞杀伤S_(180)肿瘤细胞的损伤程度小于对照组。
Studied the effect of PGE_1 on NK activity and to kill
target cell in ultrastructural feature.The results are as follows:
1,Using indirect ELISA test to detect antibody formation:PGE_1
inhibited antibody formation in serum.2,NK activity is inhibited by
PGE_1.3,Using phase contrast microscope to observe the effect of PGE_1
on NK to kill target cell.In PGE_1 group:single NK contact with target
cell,which became vacuolar degeneration,cytoplasm edema,pyknosis,
massed chromatin.In control group:more-NK contact with target cell,
which to form deformed movement.Four NK enter into target cell and
target cell became karyorrhexis,obvious pyknosis.4,Using transmissional
electron to observe that in PGE_1 group:in target cell several mitochondria
showed vacuolar degeneration,nucleus membrane sightly swelling,interval
of nucleus edema,NK extend cytoplasm protrusion insert into target cell
membrane,target cell in part destroyed,in control group:NK ruffles
attach to target cell,target cell obvious vacuolar degeneration,nucleus
membrane swelling edema,necrosis,destory.
出处
《山东医科大学学报》
1991年第2期94-97,共4页
Acta Academiae Medicinae Shandong
关键词
前列腺素E类
杀伤细胞
肿瘤细胞
Prostaglandinse
Antibodies
Killer cells,natural
Enzyme-linked immunosorbent assay
Tumor cells
Ultrastructural feature
