摘要
短串联重复序列 (STR)是由几个碱基对作为核心单位串联重复形成的一类DNA序列 ,应用 3个位点在同一反应体系中进行互不干扰的多重PCR ,采用高分辨力的聚丙烯酰胺凝胶电泳分离、银染法显影技术 ,对云南省特有的白族、傣族、阿昌族、景颇族、德昂族、拉祜族、布朗族、哈尼族、普米族和基诺族等 1 0个少数民族的F1 3A0 1、FESFPS和vWA3个位点等位基因的基因频率分布进行了调查 ,获得了满意的结果 ,在不同群体中发现一些有意义的遗传差异。
The short tandem repeats (STR) is a kind of DNA sequence formed repeatedly and connectively subjected to a core unit of several base pairs. The non interfered multi PCR was applied with 3 loci in the same reaction system. By using the technique of denaturing polyacrylamide gel electrophoresis and silver stain, the allele frequencies distributions of 3 loci: F13A01, FESFPS and vWA in 10 minority populations only resided in Yunnan Province: Bai, Dai, Achang, Jingpo, Deang, Lahu, Bulang, Hani, Pumi and Jino. A satisfactory result was obtained and some significant genetic differences were noticed in different populations.
基金
国家自然科学基金 ( 39760 0 35
399934 2 0 )
美国中华医学基金CMB项目 ( 98-675 )资助~~
