摘要
目的 :探讨碱性成纤维细胞生长因子对体外培养的中脑多巴胺能神经元的作用。方法 :在培养孕 14～16d大鼠胚胎黑质神经元中加入碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF) ,不同时间对培养细胞进行观察并计数存活的黑质细胞总数及长神经突起细胞总数。结果 :培养 1周后bFGF可促进多巴胺能神经元的存活和生长。结论
Objective: Our aim was to study the nutrient effect of basic fibroblast growth factor(bFGF)on dopaminergic (DA) neurons of the mensencephalic subtantia nigra in vitro. Methods:Subtantia nigra neurons from rats E14 to 16 were cultured with bFGF and observed by using light microscopy. Results:The bFGF can promote the development of mesencephalic DA neurons after one week. Conclusion:The bFGF has neutrient effect on DA neurons of subtantia nigra in vitro.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2001年第6期406-407,共2页
Journal of China Medical University
基金
辽宁省自然科学基金资助项目 976190 13
