摘要
金盏菊萃取物 15g ,加入 30mL正丁醇 ,密封搅拌均匀后 (5 5℃ ) ,加入 30mLw (NaOH) =2 0 %的水溶液 ,反应 7h(70℃ ) ;皂化物中加入 30 0mL水 ,搅拌均匀 ,过滤 ,然后依次用 30mL乙醇和 5 0mL正己烷冲洗 ,得到叶黄素 ,纯度为w (C4 0 H56O2 ) =71 2 %。确定了金盏菊中叶黄素的液相色谱测定方法 ,流动相为 V(CH2 Cl2 )∶V(CH3OH)∶V(CH3CN)∶V(H2 O) =32∶38∶2 9∶1,得到了叶黄素和 15种叶黄素酯的峰 ,叶黄素的保留时间为 3 6 81min ,叶黄素酯的保留时间分布为 4 5～ 12 .0min ;在流动相中它们的最大吸收波长都为 45 4nm。叶黄素的线性范围为 0 1～ 1.0 μg ,相关系数为γ =0
Marigold extraction was homogenized with butanol under sealed condition at 55°C, and then was subjected to saponification with NaOH[w (NaOH) = 20%, 30 mL] for 7 h at 70°C. The saponified product was mixed with 300 mL distilled water and filtered and the retained orange precipitate of lutein was washed with 30 mL ethanol and 50 mL n-hexane successively. The purity of resulting lutein was w (C40H56O2) = 71.2% by HPLC analysis. An improved HPLC method was developed for the analysis of lutein in marigold, the mobile phase was V (CH2Cl2):V(CH3OH): V(CH3CN):V(H2O) = 32:38:29:1 and the detecting wavelength was 454 nm. The linear range was 0.1 [similar to] 1.0 μg, γ = 0.9998.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2001年第8期467-469,共3页
Fine Chemicals
