摘要
金盏菊萃取物20g,加入正丁醇40mL,密封搅拌均匀后(55℃),加入维生素C0 2g,再加入40mLw(NaOH)=20%的水溶液在70℃进行皂化反应8h。皂化物中加入400mL水,搅拌均匀后,过滤,得到叶黄素,w(C40H56O2)=94%。将其用V(CH2Cl2)∶V(CH3OH)=2∶1结晶,叶黄素质量分数可提高至w(C40H56O2)=98%,抗氧化剂、温度、光、氧气对叶黄素的稳定性具有重要影响。
Marigold extract was homogenized with butanol (40 mL) under sealed condition at 55 ℃ and 0.2 g ascorbic acid was added.Then the mixture was subjected to saponification with 40 mL aqueous NaOH 〔w(NaOH)=20%〕 at 70 ℃ for 8 h.The saponified product was mixed with 400 mL distilled water and filtered.The purity of resulting lutein was w(C_(40)H_(56)O_2)=94%(fraction 1).Lutein was crystallized from fraction 1 with mixed solvent〔V(CH_2Cl_2)∶V(CH_3OH)=2∶1〕to raise the purity to 98%.Antioxidant,temperature,light and oxygen have important influence on the stability of lutein.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2004年第6期447-449,共3页
Fine Chemicals
基金
山东省优秀中青年科学家奖励基金(2000)~~
关键词
叶黄素
分离
纯化
结晶
稳定性
lutein
isolation
purification
crystallization
stability
