摘要
目的 :为了从形态学方面更准确、更客观地鉴别胆囊良恶性病变 ,对胆囊良恶性细胞进行体视学定量分析。方法 :运用方网测试系统在显微镜下测量了 2 2例胆囊良性病变及 2 2例胆囊癌石蜡标本切片细胞的体视学参数 :核体积密度 (Vvn)、核表面积密度 (Svn)、核表面积体积比 (Rsvn)、核浆比 (Rnp)、核长轴 (a)、短轴 (b)及轴比 (a/b)。结果 :胆囊恶性病变细胞体视学参数Vvn、Svn、Rnp、a和b明显大于良性病变 ,它们之间均有显著性差异 (P <0 .0 5 ) ,而Rsvn和a/b由于恶性细胞核偏向球形生长 ,虽然它的表面积增大及长轴较良性病变长 ,但其之比差异无显著性意义 (P >0 .0 5 )。在腺癌中 ,病理Ⅰ级与Ⅲ级 ,无转移与转移的Vvn和Rnp参数差异有显著性意义 (P <0 .0 5 )。在良性病变中 ,腺瘤与胆固醇息肉、单纯性增生的Vvn、Rnp也存在显著性差异 (P <0 .0 5 )。结论 :胆囊恶性细胞核增大、变圆 ,Vvn、Rnp两个参数是细胞核异型性的重要指标 ,且为临床形态学诊断提供了依据。
Objective:To analyze stereologically the data of the malignant and benign lesions of gallbladder.Methods:22 specimens of gallbladder cancer and 22 specimens of benign lesion of gallbladder were measured with squard net detective system under microscope. The data for the steredogical analysis include volumn density of nuclei,surface area density of nuclei(Svn), ratio of surface area and volumn of nuclei(Rsvn), ratio of nuclei and plasma(Rnp), nuclei long axis. Short axis and ratio of axis.Results:Those stereological data of Vvn?Svn?Rnp?aAnd b of cells with malignant were significantly greater than those with benign lesion(P<0.05). There were no obvious differences of Rsvn and a/b between cells with malignant lesion and cells with benign lesion(P>0.05). The Vvn and Rnp of grade Ⅰ were significantly different from those of grade Ⅲ and the Vvn and Rnp of no metastasis were also significantly different from those of metastasis in gland cancer(P<0.05). The results also showed that the Vvn and Rnp were significantly different between adenoma and cholesterin polyps and between adenoma and simply hyperplasia(P<0.05).Conclusion:The analysis of stereology make the discerning of the malignant and benign lesions of gallbladder more objective and comparable. The Vvn and Rnp were important indexes of cellular abnormity,which provides support for clinical morphological diagnosis.
出处
《肝胆胰外科杂志》
CAS
2001年第4期178-181,共4页
Journal of Hepatopancreatobiliary Surgery
基金
江西省科委资助项目
关键词
胆囊
良恶性病变
细胞
体视学
gallbladder
malignant and benign lesion
cells
stereology
