摘要
用电击转化法将质粒pBI12 1(含GUS基因 ,β 葡萄糖苷酸酶基因 )导入苜蓿愈伤组织的小细胞团内 。
Alfalfa calli were induced and obtained from organ of sterile seedlings such as cotyledon,hypocolyl,stem and root by tissue culture technique. Small cell clusters of alfalfa calli were prepared after calli subculture,and plasmid pBI121 which contains a copy of GUS gene(β-glucurcnidase,reported gene)was introduced into alfalfa calli cell clusters by electroporation(by gene pulser).Transient expression were determined and discovered in these receptor calli cells by histochemical localization reaction.This work would explore out a new technique for transgenic alfalfa research.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第6期905-908,共4页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金 (39980 0 31)
