摘要
作者以成单玉米幼胚经诱导、继代、筛选 ,获得了可分化的胚性愈伤组织 ,采用电击转化法 ,将质粒pBI12 1导入成单玉米的胚性愈伤组织中 ,通过组织化学染色法 ,观察到了GUS基因的瞬时表达 ,并筛选出对愈伤组织损害轻、GUS表达较强的最适电击条件为 :96 0 μF ,375V/cm ,6 1ms.
This thesis firstly deals with embrogenic calli by inducing, continuously culturing and selecting from immature embryos of Chengdan maize. The plasmid of pBI121 was transfected into embrogenic calli of Chengdan maize by electroporation. The transient expression dots of GUS gene were observed using coloration of culture chemistry. The best condition of electroporation was 960μF,375V/cm,61ms.The results show that it is harmless for embrogenic calli and expression stronger of GUS in this condition.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第6期913-918,共6页
Journal of Sichuan University(Natural Science Edition)
基金
四川省重点项目基金
国家自然科学基金 (39780 0 18)
国家转基因植物及产业化专项基金 (J0 0 A 0 0 8 0 9)
