摘要
基于抗坏血酸还原Fe(?)-1-(2-吡定偶氮)-2-萘酚-6-磺酸犤简写为Fe(Ⅲ)-PAN-S犦为Fe(Ⅱ)-PAN-S,建立了分光光度法测定水果中抗坏血酸的方法。在pH4.5的HAc--NaAc缓冲溶液中,Fe(Ⅱ)-PAN-S络合物的最大吸收峰位于549nm和748nm处。在748nm处,抗坏血酸含量在0-100μg/25ml)范围内符合比耳定律,方法操作简便,选择性较高,可用于水果中抗坏血酸的测定。
As ascorbic acid can rdeuce Fe(Ⅲ)-PAN-S to Fe(Ⅱ)-PAN-S.We used spectriphotmetic method to detiermine ascorbic acid of the fruits in this paper.Fe(Ⅱ)-PAN-S comple has main high peaks at 549nm and 748nm in HAc-NaAc buffer solution at pH=4.5.At 748nm,the content of ascorbic acid (in 0-100μg/25ml)is just in accordance with Beer's law.It is very convenience and has high selection using this method,especially for the determination of ascorbic acid of the fruits.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第12期59-61,共3页
Food Science
基金
浙江省分析测试基金资助项目.项目编号:00080
