摘要
综述了植酸酶基因工程的研究进展 ,着重介绍了一种颇具前途的细菌植酸酶基因appA及其过量表达方面的研究 ,并对该酶的酶学性质特别是其在动物生理条件下所表现出的性质进行了初步总结。讨论了当前植酸酶研究中存在的一些困难 ,并对其进一步研究进行了展望。
The appA gene previously shown to encode for an acid phosphatase actually codes for a bifunctional enzyme exhibiting both acid phosphatase activities. According to the researches on this enzyme, the mass molecular was relatively low and the effective activity was relatively high compared with those of the fungal phytase. The enzyme had identical optimal pH of 4.5 for the phytase activity and was stable at pH value from 2 -10. When heated at different pH values the enzyme showed desired thermal resistance. Furthermore the enzyme resisted the protease at the physiological conditions of common livestock. These properties made the enzyme a promising candidate for industrial production of feed enzyme. In this paper, the discovery and overexpression of this gene were introduced in detail and the research trend was discussed as well.KEYWARDS phytase; acid phosphatase; Escherichia coli; appA gene; genetic engineering
出处
《粮食与饲料工业》
CAS
2001年第10期32-34,共3页
Cereal & Feed Industry
