摘要
目的 探讨基质金属蛋白酶 (MMPS)抑制剂对视网膜新生血管化抑制作用的应用价值。方法 将新生 7天的C5 7BL/ 6J小鼠暴露 75 %高氧中 5天 ,再置于一般空气中 5天 ,造成视网膜新生血管化的动物模型 ,分成 3组 ,其中1组为对照组。实验组中 1组给予Batimastat腹腔注射 ,另 1组注射生理盐水作对照。用免疫组化染色 ,并用显微镜下微血管计数方法研究视网膜新生血管及其使用MMPS抑制剂的情况。同时采用酶谱法 ,分析MMPS在各组中含量的变化。结果 注射Batimastat的试验组微血管的数量明显低于注射生理盐水的对照组 ,同时MMPS的表达也减少 ,具有统计学显著性差异。结论 Batimastat作为一种新的MMPS抑制剂可以抑制视网膜新生血管化 ,并减低MMPS的表达。
ObjectiveTo investigate the therapeutic effects of the matrix metalloproteinases (MMPS) inhibitor on the animal model of ischemicinduced retinal neovascularization.MethodsRetina neovascularization was induced in newborn mice exposed.to 75% oxygen for five days,followed by room air,the mices were subdivided into three groups,one group was used as control,the others received intraperitoneal injections of a MMPS inhibitor,histological analysis was done to quantitate the neovascular response in these animals.Retinal extracts underwent zymographic analysis to measure the activity of MMPS.ResultsLevels of MMPS (MMP 2,MMP 9) in retina were significantly increased in animals with induced retina neovascularizaton.retina neovascularization was significantly inhibited with intraperitoneal of a MMP inhibitor.ConclusionA MMP inhibitor may have therapeutic potential in preventing retina neovascularization.
出处
《眼科研究》
CSCD
北大核心
2001年第6期511-514,共4页
Chinese Ophthalmic Research
