摘要
目的探讨荧光定量PCR法检测乙肝病毒的临床意义。 方法荧光定量PCR法检测 15 4例患者的乙肝病毒量 ,研究其与乙肝病毒抗体 ,谷丙转氨酶 (ALT)及反映肝纤维化指标的透明质酸酶 (HA)与层粘蛋白 (LN)的关系。 结果免疫荧光定量PCR方法直接测定病毒量 ,明确反映病毒感染程度 ,其测定值与ALT呈一定程度正相关 ,与肝炎轻、中、重程度及肝纤维化程度成反比。
Objective To discuss the clinical significance of detecting HBV-DNA by fluorescent PCR. Methods To study the relationship between the quantity of HBV-DNA and HBV-M, ALT, HA, LN by detecting in 154 cases. Results The fluorescent PCR method can direltly detect the quantity of HBV-DNA. The detection bears positive relationship with ALT, and negative relationship with the severity of hepatitis liver fibrosis. Conclusion We can estimate the severity of hepatitis by this method and conduct appropriate anti-virus and antifibrosis therapy.
出处
《上海第二医科大学学报》
CSCD
2001年第4期372-373,共2页
Acta Universitatis Medicinalis Secondae Shanghai
