摘要
根据已报道的哺乳动物GH基因序列设计一对引物 ,利用PCR技术直接扩增猞猁GH基因部分序列和 5′端调控区序列 ,将PCR产物克隆到质粒pUC19的HincⅡ位点 ,重组子经HindⅢ /EcoRⅠ双酶切鉴定和序列分析 ,扩增片段长度为 783bp。测序结果与其它报道的哺乳动物GH基因对应片段比较 ,5′端侧翼序列和第一外显子高度保守 ,第一内含子和部分第二内含子存在 89%同源性 ,且第二个外显子中突变多发生在密码子的兼并位点上。PCR结果和序列分析表明 。
The partial growth hormone (GH) gene and its 5′flanking regulatory region of Felis lynx was amplified by PCR with a pair of primes based on the sequences of the reported mammalian GH gene for the first time. The PCR products were cloned on Hinc Ⅱ sites of plasmid pUC19. It was determined that its length is 783 bp by the enzymes digesting and sequencing. Comparing the corresponding sequences of GH gene among Felis lynx with the other reported mammal′s, the authors concluded that there was high conservation in the 5′flanking sequence and exonⅠ, and exsited 89% homologus in the intronⅠ and the partial intronⅡ. Many variations of the exonⅡ occurred in the degenerate sites of the triplet code. The results of PCR shows that the GH gene of Felis lynx is a single gene in the genome.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2001年第5期103-106,共4页
Journal of Northeast Forestry University
基金
国家自然科学基金 (No .39970 10 3)资助
