摘要
探讨二咖啡酰奎宁酸 (IBE5 )对成纤维细胞活力 ,增殖及功能的影响 .研究 IBE5抗肝纤维化的机制 .实验采用体外培养的 NIH/ 3T3细胞作为成纤维细胞的替代模型 ,常规培养 .质量浓度为 2 5 0 ,12 5 ,6 2 .5 ,31.2 5 ,15 .6 ,7.8mg/ L 的 IBE5加入细胞中培养 2 4h;[3 H]- Td R掺入法测定细胞增殖率 ;[3 H]- Pro掺入法测定细胞活力 ;[3 H]- Pro掺入 ,胶原酶消化法测定细胞内外胶原生成率 .结果显示以上 6个浓度的 IBE5对细胞均无毒性 .各组均可显著抑制细胞增殖 ,促进细胞活力 ,明显抑制胶原和透明质酸的合成 ,以 2 5 0 m g/ L 为最佳浓度 .IBE5能显著抑制 3T3细胞增殖 ,胶原和透明质酸合成并对细胞活力有促进作用 .IBE5在体外具有显著的抗肝纤维化作用 。
The effects of Dicaffeoylquinic Acid (IBE5) on proliferation, viability, function of fibroblasts, and the mechanism of protecting against fibrosis on liver were studied. As the substitutive model of liver fibroblasts, NIH/3T3 fibroblasts were cultured with regular method in vitro , and incubated with 250,125,62.5,31.25,15.6,7.8 mg/L IBE5 for 24 hours. The cell viability and hylturonic acid level was assayed by 3H] Pro incorporation, cell proliferation by Pro incorporation, cell proliferation by 3H] TdR incorporation. Cell collagen synthesis rate was measured with TdR incorporation. Cell collagen synthesis rate was measured with 3H] Pro incorporation collagenase digestion. The results showed that the addition of IBE5 (7.8～250 mg/L) significantly reduced proliferation, inhibited intracellular cell viability and decreased the hyaluronic acid level in dose dependent manner. 250 mg/L was the best dose. The IBE5 has with notable antifibrotic function and could be applied for anti liver fibrosis.
出处
《生命科学研究》
CAS
CSCD
2001年第4期355-357,共3页
Life Science Research
基金
国家自然科学基金资助项目 ( 396 2 2 0 0 3)
