摘要
为探讨端粒酶反义寡核苷酸对人乳腺癌 MCF- 7细胞端粒酶活性及细胞生长的影响 ,应用与人端粒酶 RNA组分 ( h TR)模板区互补的硫代反义寡核苷酸处理 MCF- 7细胞。发现经反义 h TR寡核苷酸作用后 ,细胞端粒酶活性明显受到抑制 ,至作用后 72 h,端粒酶活性较对照组降低 61.0 % ( P<0 .0 5 ) ;细胞生长明显受到抑制 ;细胞周期发生显著变化 ,G0 / G1 期细胞数增多 ,S期及 G2 / M期细胞数则减少 ;细胞增殖指数由 0 .4 6降低至 0 .2 9;并可观察到凋亡细胞的出现 ,凋亡发生率为 10 .7% ;而无义寡核苷酸处理组及空白对照组以上各指标均无明显变化。提示 :端粒酶反义寡核苷酸可明显抑制乳腺癌细胞端粒酶活性 ,抑制细胞生长和增殖 ,诱导细胞凋亡 ;表明应用反义技术抑制端粒酶活性治疗乳腺癌可能具有广阔的前景。
In order to investigate the effects of telomerase antisense oligodeoxynucleotides (ODNs) on telomerase activity and the growth of breast cancer cells, MCF-7 cells were treated with antisense phosphorothoate oligodeoxynucleotides complementary to the template region of human telomerse RNA (hTR). The results showed that telomerase activity were suppressed significantly, and decreased by 61 0 % after 72 h as compared with control group (P<0 05); The growth of MCF-7cells were inhibited significantly; Cell cycle phases were altered significantly: S phase and G 2 /M phase cells were decreased and G 0 /G 1 phase cells were increased, the proliferation index decreased from 0 46 to 0 29; Apoptosis cells were observed after 120 h, and apoptosis rate was 10 7 %. However, in the groups of nosense ODNs and control the above parameters were not changed significantly (P>0 05). The results suggest that telomerse antisense oligodeoxynucleotides can significantly suppress the telomerse activity, inhibit cell growth and cell proliferation, and induce cell apoptosis of MCF-7 cells. It may be possible to control breast cancer by inhibiting telomerse activity through antisense technique.
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第5期428-430,共3页
Acta Universitatis Medicinae Tongji
