摘要
目的探讨端粒粒酶反义寡核苷酸对肺癌细胞端粒酶活性的抑制作用。方法脂质体介导的端粒酶反义寡核苷酸转染A549肺腺癌细胞株,采用TRAP-PCR-ELISA法半定量检测转染后肺腺癌细胞端粒酶活性,观察它们的变化。结果端粒酶反义寡核苷酸转染A549细胞72h后,A549细胞端粒酶活性明显下调。结论端粒酶反义寡核苷酸可以有效抑制肺腺癌细胞端粒酶活性。
Objective:To study the inhibitory effect of telomerase antisense oligodeoxynucleotide on telomerase activity in lung carcinoma cells.Methods:Phosphorothioate antisense oligodeoxynucleotide of telomerase was transfected into A549cell lines by lipofectamine(LP).72hours later,the change of telomerae activity was assayed by TRAP-PCR-ELISA.Results:72hours after transfecting antisense oligodeoxynucleotide into A549cell lines,telomerase activity was suppressed significantly.Conclusions:Antisense oligodeoxynu-cleotide targeting at telomerase can inhibit telomerase activity of lung adenocarcinoma cells effectively.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第11期14-17,共4页
China Journal of Modern Medicine
基金
TheresearchprojectfundedbytheNationalNaturalFounda-tionofHunanProvince.(No.03JJY4040)
